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Markus Sperandio, MD

Markus Sperandio, MD

Walter-Brendel-Center of Experimental Medicine
Ludwig-Maximilians-Universität
Marchioninistr. 27
81377 München
Germany
+49 89-2180 76505
+49 89-2180 76503

Email: markus.sperandio@lmu.de

Website: http://www.sfb914.med.uni-muenchen.de/

 

Our lab investigates the molecular mechanisms of leukocyte recruitment using intravital microscopy and several static and dynamic in vitro assays. Leukocyte recruitment proceeds along a well-defined cascade of events starting with selectin-dependent leukocyte capture to and rolling along the vessel wall. During rolling, leukocytes get into intimate contact with the endothelium, which allows endothelial-bound chemokines to interact with specific chemokine-receptors on leukocytes. This in turn triggers the activation of integrins leading to firm arrest followed by transmigration into tissue.

We are interested in all aspects of leukocyte recruitment during inflammation in vivo. This includes pattern recognition receptors, the role of posttranslational glycosylation on selectin ligand, chemokine-receptor function and integrin activation. In addition, we work with a intravital microscopy model in the mouse fetus, which provides us with a powerful tool to investigate the maturing immune system during ontogeny in different embryonic and extraembryonic compartments.

 

Group members
Postdocs: Monika Prünster, PhD; Kristina Heinig, PhD; Claudia Nussbaum, MD
PhD students: Angela Kurz, Roland Immler
MD students: Tobias Steffen Annamaria Florian
Technicians: Susanne Bierschenk, Nadine Schmidt

 

Recent selected publications
Klapproth S, Moretti FA, Zeiler M, Ruppert R, Breithaupt U, Mueller S, Haas R, Mann M, Sperandio M, Fässler R, Moser M. Minimal amounts of kindlin-3 suffice for basal platelet and leukocyte functions in mice. Blood. 2015 Dec 10;126(24):2592-600

Klapproth S, Sperandio M, Pinheiro EM, Prünster M, Soehnlein O, Gertler FB, Fässler R, Moser M. Loss of the Rap1 effector RIAM results in leukocyte adhesion  deficiency due to impaired β2 integrin function in mice. Blood. 2015 Dec 17;126(25):2704-12.

Ruppert R, Moser M, Sperandio M, Rognoni E, Orban M, Liu WH, Schulz AS, Oostendorp RA, Massberg S, Fässler R. Kindlin-3-mediated integrin adhesion is dispensable for quiescent but essential for activated hematopoietic stem cells. J Exp Med. 2015 Aug 24;212(9):1415-32.

Heinig K, Sage F, Robin C, Sperandio M. Development and trafficking function of haematopoietic stem cells and myeloid cells during fetal ontogeny. Cardiovasc  Res. 2015 Aug 1;107(3):352-63.

Pruenster M, Kurz AR, Chung KJ, Cao-Ehlker X, Bieber S, Nussbaum CF, Bierschenk S, Eggersmann TK, Rohwedder I, Heinig K, Immler R, Moser M, Koedel U,  Gran S, McEver RP, Vestweber D, Verschoor A, Leanderson T, Chavakis T, Roth J, Vogl T, Sperandio M. Extracellular MRP8/14 is a regulator of β2 integrin-dependent neutrophil slow rolling and adhesion. Nat Commun. 2015 Apr 20;6:6915.

Nussbaum C, Bannenberg S, Keul P, Gräler MH, Gonçalves-de-Albuquerque CF, Korhonen H, von Wnuck Lipinski K, Heusch G, de Castro Faria Neto HC, Rohwedder I, Göthert JR, Prasad VP, Haufe G, Lange-Sperandio B, Offermanns S, Sperandio M, Levkau B. Sphingosine-1-phosphate receptor 3 promotes leukocyte rolling by mobilizing endothelial P-selectin. Nat Commun. 2015 Apr 2;6:6416.

Chung KJ, Mitroulis I, Wiessner JR, Zheng YY, Siegert G, Sperandio M, Chavakis T. A novel pathway of rapid TLR-triggered activation of integrin-dependent leukocyte adhesion that requires Rap1 GTPase. Mol Biol Cell. 2014 Oct 1;25(19):2948-55.

Weckbach LT, Gola A, Winkelmann M, Jakob SM, Groesser L, Borgolte J, Pogoda F, Pick R, Pruenster M, Müller-Höcker J, Deindl E, Sperandio M, Walzog B. The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via β2 integrins (CD11/CD18). Blood. 2014 Mar 20;123(12):1887-96.

Döring Y, Noels H, Mandl M, Kramp B, Neideck C, Lievens D, Drechsler M, Megens RT, Tilstam PV, Langer M, Hartwig H, Theelen W, Marth JD, Sperandio M, Soehnlein O, Weber C. Deficiency of the sialyltransferase St3Gal4 reduces Ccl5-mediated myeloid cell recruitment and arrest: short communication. Circ Res. 2014 Mar 14;114(6):976-81.

Ziegler T, Horstkotte J, Schwab C, Pfetsch V, Weinmann K, Dietzel S, Rohwedder I, Hinkel R, Gross L, Lee S, Hu J, Soehnlein O, Franz WM, Sperandio M,  Pohl U, Thomas M, Weber C, Augustin HG, Fässler R, Deutsch U, Kupatt C. Angiopoietin 2 mediates microvascular and hemodynamic alterations in sepsis. J Clin Invest. 2013 Jul 1. pii: 66549.

Sperandio M, Quackenbush EJ, Sushkova N, Altstätter J, Nussbaum C, Schmid S,  Pruenster M, Kurz A, Margraf A, Steppner A, Schweiger N, Borsig L, Boros I, Krajewski N, Genzel-Boroviczeny O, Jeschke U, Frommhold D, von Andrian UH. Ontogenetic regulation of leukocyte recruitment in mouse yolk sac vessels. Blood. 2013 May 23;121(21):e118-28.

Jakob SM, Pick R, Brechtefeld D, Nussbaum C, Kiefer F, Sperandio M, Walzog B. Hematopoietic progenitor kinase 1 (HPK1) is required for LFA-1-mediated neutrophil recruitment during the acute inflammatory response. Blood. 2013 May 16;121(20):4184-94.

Schmidt S, Moser M, Sperandio M. The molecular basis of leukocyte recruitment and its deficiencies. Mol Immunol. 2013 Aug;55(1):49-58.

Nussbaum C, Gloning A, Pruenster M, Frommhold D, Bierschenk S, Genzel-Boroviczény O, von Andrian UH, Quackenbush E, Sperandio M. Neutrophil and  endothelial adhesive function during human fetal ontogeny. J Leukoc Biol. 2013 Feb;93(2):175-84.

Nussbaum C, Klinke A, Adam M, Baldus S, Sperandio M. Myeloperoxidase: a leukocyte-derived protagonist of inflammation and cardiovascular disease. Antioxid Redox Signal. 2013 Feb 20;18(6):692-713.

Yang WH, Nussbaum C, Grewal PK, Marth JD, Sperandio M. Coordinated roles of ST3Gal-VI and ST3Gal-IV sialyltransferases in the synthesis of selectin ligands.  Blood. 2012 Aug 2;120(5):1015-26.

Nussbaum C, Sperandio M. Innate immune cell recruitment in the fetus and neonate. J Reprod Immunol. 2011 Jun;90(1):74-81.

Frommhold D, Kamphues A, Hepper I, Pruenster M, Lukic IK, Socher I, Zablotskaya V, Buschmann K, Lange-Sperandio B, Schymeinsky J, Ryschich E, Poeschl J, Kupatt C, Nawroth PP, Moser M, Walzog B, Bierhaus A, Sperandio M. RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in  vivo. Blood. 2010 Aug 5;116(5):841-9.  Sperandio M, Gleissner CA, Ley K. Glycosylation in immune cell trafficking. Immunol Rev. 2009 Jul;230(1):97-113.

Moser M, Bauer M, Schmid S, Ruppert R, Schmidt S, Sixt M, Wang HV, Sperandio  M, Fässler R. Kindlin-3 is required for beta2 integrin-mediated leukocyte adhesion to endothelial cells. Nat Med. 2009 Mar;15(3):300-5

Frommhold D, Ludwig A, Bixel MG, Zarbock A, Babushkina I, Weissinger M, Cauwenberghs S, Ellies LG, Marth JD, Beck-Sickinger AG, Sixt M, Lange-Sperandio B, Zernecke A, Brandt E, Weber C, Vestweber D, Ley K, Sperandio M. Sialyltransferase ST3Gal-IV controls CXCR2-mediated firm leukocyte arrest during  inflammation. J Exp Med. 2008 Jun 9;205(6):1435-46.